Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary Targetcam kinase 2

Cell permeable: yes

General description

A cell-permeable, competitive inhibitor of Ca²⁺/calmodulin-dependent protein kinase II (K_i = 370 nM). Selectively binds to the Calmodulin (CaM) binding site of the enzyme and prevents the association of CaM with CaM Kinase II. Inhibits glucose-induced and forskolin-stimulated insulin release from isolated pancreatic islets. Has no significant effect on protein kinase A activity. Induces G₁ cell cycle arrest and apoptosis in NIH 3T3 cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Fan, G.H., et al. 1999. Mol. Pharmacol.56, 39.Tombes, R.M., et al. 1995. Cell Growth Differentiation6, 1063.Chen, C., et al. 1994. Science266, 291.Mamiya, N., et al. 1993. Biochem. Biophys. Res. Commun.195, 608.Niki, I., et al. 1993. Biochem. Biophys. Res. Commun.191, 255.Sumi, M., et al. 1991. Biochem. Biophys. Res. Commun.181, 968.Tokumitsu, H., et al. 1990. J. Biol. Chem.265, 4315.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

A 5 mM (1 mg/399 µl) solution of KN-93 (Cat. No. 422708) in DMSO.

Reconstitution

Following initial thaw aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)